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5x first strand buffer  (Thermo Fisher)


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    Thermo Fisher 5x first strand buffer
    5x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/first+strand+buffer+5x/TRIS-HCL/pm41628766-56-27-51
    Average 99 stars, based on 1 article reviews
    5x first strand buffer - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Transcriptomic, clonal, and functional analyses reveal Liver tissue-imprinted immuno-profile of circulating autoreactive CD4 T cells in autoimmune liver diseases
    Article Snippet: Reverse transcription was performed using 2μg of total RNA incubated at 70°C for 10min with poly-dT24 20μg/mL (Eurofins Genomics), 8mM DTT (18057018, ThermoFisher Scientific) and 20mM of each dNTP (10297018, ThermoFisher Scientific). .. After a brief 5min incubation at 4°C, first strand buffer 5X (Y00146, Invitrogen), 200U of M-MLV reverse transcriptase (18057018, ThermoFisher Scientific) and 40U of RNAse OUT inhibitor (10777019, Invitrogen) were added and incubated at 37°C for 1h followed by 15min at 70°C. .. Real-time quantitative PCR was performed using the ViiA TM 7 Real-Time PCR System and Power SYBR TM Green PCR Master Mix (4368708, ThermoFisher Scientific).

    Article Title: Accumulation of PD-1+ TIGIT+ T cells in the liver after local antigen reactivity and during autoimmune hepatitis
    Article Snippet: Reverse transcription was performed using 2μg of total RNA incubated at 70°C for 10min with poly-dT24 20μg/mL (Eurofins Genomics), 8mM DTT (18057018, ThermoFisher Scientific) and 20mM of each dNTP (10297018, ThermoFisher Scientific). .. After a brief 5min incubation at 4°C, first strand buffer 5X (Y00146, Invitrogen), 200U of M-MLV reverse transcriptase (18057018, ThermoFisher Scientific) and 40U of RNAse OUT inhibitor (10777019, Invitrogen) were added and incubated at 37°C for 1h followed by 15min at 70°C. .. Real-time quantitative PCR was performed using the ViiA 7 Real-Time PCR System and Power SYBR Green PCR Master Mix (4368708, ThermoFisher Scientific).

    Article Title: Single cell profiling of circulating autoreactive CD4 T cells from patients with autoimmune liver diseases suggests tissue imprinting
    Article Snippet: For plasmid CMV HA vector, HA sequence (Influenza A virus (A/Puerto Rico/8/1934(H1N1), Gene ID: 956529) was synthesized (GenScript, NETHERLANDS) and cloned in pCMVβ vector (Clontech Laboratories) in place of β-galactosidase gene. .. After a brief 5 min incubation at 4 °C, first strand buffer 5X (Y00146, Invitrogen), 200U of M-MLV reverse transcriptase (18057018, ThermoFisher Scientific), and 40U of RNAse OUT inhibitor (10777019, Invitrogen) were added and incubated at 37 °C for 1 h followed by 15 min at 70 °C. .. Depletion of CD4 + cells was performed by i.p injection of 500 μg of anti-mouse CD4 antibody, using purified Rat IgG2b,κ isotype control antibody, every two days during 2 weeks.

    Article Title: Single cell profiling of circulating autoreactive CD4 T cells from patients with autoimmune liver diseases suggests tissue imprinting.
    Article Snippet: Reverse transcriptionwasperformedusing 2 μgof total RNA incubated at 70 °C for 10min with poly-dT24 20 μg/mL (Eurofins Genomics), 8mM DTT (18057018, ThermoFisher Scientific), and 20mM of each dNTP (10297018, ThermoFisher Scientific). .. After a brief 5min incubation at 4 °C, first strand buffer 5X (Y00146, Invitrogen), 200U of M-MLV reverse transcriptase (18057018, ThermoFisher Scientific), and 40U of RNAse OUT inhibitor (10777019, Invitrogen) were added and incubated at 37 °C for 1 h followed by 15min at 70 °C. .. Induction of HA expression by hepatocytes was performed by feeding mice with tamoxifen dry food (0.5 g/kg tamoxifen + 5% saccharose; Safe, FRANCE) for 14 days in free access or by injecting intraperitonially (i.p) 1mg of tamoxifen (T5648, Sigma) diluted in corn oil (C8267, Sigma) three times in two weeks span.

    Reverse Transcription:

    Article Title: Transcriptomic, clonal, and functional analyses reveal Liver tissue-imprinted immuno-profile of circulating autoreactive CD4 T cells in autoimmune liver diseases
    Article Snippet: Reverse transcription was performed using 2μg of total RNA incubated at 70°C for 10min with poly-dT24 20μg/mL (Eurofins Genomics), 8mM DTT (18057018, ThermoFisher Scientific) and 20mM of each dNTP (10297018, ThermoFisher Scientific). .. After a brief 5min incubation at 4°C, first strand buffer 5X (Y00146, Invitrogen), 200U of M-MLV reverse transcriptase (18057018, ThermoFisher Scientific) and 40U of RNAse OUT inhibitor (10777019, Invitrogen) were added and incubated at 37°C for 1h followed by 15min at 70°C. .. Real-time quantitative PCR was performed using the ViiA TM 7 Real-Time PCR System and Power SYBR TM Green PCR Master Mix (4368708, ThermoFisher Scientific).

    Article Title: Accumulation of PD-1+ TIGIT+ T cells in the liver after local antigen reactivity and during autoimmune hepatitis
    Article Snippet: Reverse transcription was performed using 2μg of total RNA incubated at 70°C for 10min with poly-dT24 20μg/mL (Eurofins Genomics), 8mM DTT (18057018, ThermoFisher Scientific) and 20mM of each dNTP (10297018, ThermoFisher Scientific). .. After a brief 5min incubation at 4°C, first strand buffer 5X (Y00146, Invitrogen), 200U of M-MLV reverse transcriptase (18057018, ThermoFisher Scientific) and 40U of RNAse OUT inhibitor (10777019, Invitrogen) were added and incubated at 37°C for 1h followed by 15min at 70°C. .. Real-time quantitative PCR was performed using the ViiA 7 Real-Time PCR System and Power SYBR Green PCR Master Mix (4368708, ThermoFisher Scientific).

    Article Title: Single cell profiling of circulating autoreactive CD4 T cells from patients with autoimmune liver diseases suggests tissue imprinting
    Article Snippet: For plasmid CMV HA vector, HA sequence (Influenza A virus (A/Puerto Rico/8/1934(H1N1), Gene ID: 956529) was synthesized (GenScript, NETHERLANDS) and cloned in pCMVβ vector (Clontech Laboratories) in place of β-galactosidase gene. .. After a brief 5 min incubation at 4 °C, first strand buffer 5X (Y00146, Invitrogen), 200U of M-MLV reverse transcriptase (18057018, ThermoFisher Scientific), and 40U of RNAse OUT inhibitor (10777019, Invitrogen) were added and incubated at 37 °C for 1 h followed by 15 min at 70 °C. .. Depletion of CD4 + cells was performed by i.p injection of 500 μg of anti-mouse CD4 antibody, using purified Rat IgG2b,κ isotype control antibody, every two days during 2 weeks.

    Article Title: Altered placental expression of neurotransmitter and stress-related molecules in first-episode psychosis during pregnancy: Implications for cytoarchitecture and function.
    Article Snippet: .. A reverse transcription reaction mix was prepared, containing 2.8 μL of First Strand Buffer 5X (Thermo Fisher Scientific), 1 μL of reverse transcriptase enzyme, 2 μL of 10 mM dNTP mix, 2 μL of 0.1 M DTT, 1.7 μL of nuclease-free water, and 0.5 μL of RNase inhibitor. ..

    Article Title: Estradiol affects the expression of essential molecular factors involved in luteinizing hormone secretion in the plains vizcacha.
    Article Snippet: The plains vizcacha is a rodent that shows reactivation of the hypothalamic–pituitary–ovary (HPO) axis activity at mid-gestation.. This process is enabled by the secretion of hypothalamic gonadotropin-releasing hormone (GnRH) at mid-gestation, followed by follicle-stimulating hormone (FSH) and luteinizing hormone (LH) secretion.. However, a decrease in the pituitary GnRH receptor (GnRHR) expression is concomitantly determined.

    Article Title: Single cell profiling of circulating autoreactive CD4 T cells from patients with autoimmune liver diseases suggests tissue imprinting.
    Article Snippet: Reverse transcriptionwasperformedusing 2 μgof total RNA incubated at 70 °C for 10min with poly-dT24 20 μg/mL (Eurofins Genomics), 8mM DTT (18057018, ThermoFisher Scientific), and 20mM of each dNTP (10297018, ThermoFisher Scientific). .. After a brief 5min incubation at 4 °C, first strand buffer 5X (Y00146, Invitrogen), 200U of M-MLV reverse transcriptase (18057018, ThermoFisher Scientific), and 40U of RNAse OUT inhibitor (10777019, Invitrogen) were added and incubated at 37 °C for 1 h followed by 15min at 70 °C. .. Induction of HA expression by hepatocytes was performed by feeding mice with tamoxifen dry food (0.5 g/kg tamoxifen + 5% saccharose; Safe, FRANCE) for 14 days in free access or by injecting intraperitonially (i.p) 1mg of tamoxifen (T5648, Sigma) diluted in corn oil (C8267, Sigma) three times in two weeks span.

    other:

    Article Title: Antiviral activity of intracellular nanobodies targeting the influenza virus RNA-polymerase core
    Article Snippet: After 5 min, 14.5 μL of a preheated reaction mix (4 μl First Strand buffer 5X (Thermo Fisher), 1 μL 0.1 M dithiothreitol (DTT), 1μL dNTP mix (10 mM each), 1μL Superscript III reverse transcriptase (200 U/μl, Thermo Fisher), 1 μL RNasin Plus RNase inhibitor (40 U/μL, Promega) and 6.5 μL saturated trehalose) was added and incubated at 60°C for 1 h. Real-time PCR (qPCR) was performed with the iTaqTM Universal SYBR® Green Supermix (BioRad) on a CFX96 (BioRad).

    Article Title: Antiviral activity of intracellular nanobodies targeting the influenza virus RNA-polymerase core
    Article Snippet: After 5 min, 14.5 μL of a preheated reaction mix (4 μl First Strand buffer 5X (Thermo Fisher), 1 μL 0.1 M dithiothreitol (DTT), 1μL dNTP mix (10 mM each), 1μL Superscript III reverse transcriptase (200 U/μl, Thermo Fisher), 1 μL RNasin Plus RNase inhibitor (40 U/μL, Promega) and 6.5 μL saturated trehalose) was added and incubated at 60°C for 1 h. Real-time PCR (qPCR) was performed with the iTaq Universal SYBR Green Supermix (BioRad) on a CFX96 (BioRad).

    Random Hexamer:

    Article Title: Estradiol affects the expression of essential molecular factors involved in luteinizing hormone secretion in the plains vizcacha.
    Article Snippet: The plains vizcacha is a rodent that shows reactivation of the hypothalamic–pituitary–ovary (HPO) axis activity at mid-gestation.. This process is enabled by the secretion of hypothalamic gonadotropin-releasing hormone (GnRH) at mid-gestation, followed by follicle-stimulating hormone (FSH) and luteinizing hormone (LH) secretion.. However, a decrease in the pituitary GnRH receptor (GnRHR) expression is concomitantly determined.



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